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KMID : 0900920020260040377
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Korean journal of Animal Reproduction
2002 Volume.26 No. 4 p.377 ~ p.384
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Protein Profile of Embryonic Stem Cells Following Differentiation
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Jin D.-I.
Lee S.-H.
Park C.-S.
Im K.-S.
Lee H.-M.
Kang H.-S.
Kim H.-J.
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Abstract
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To investigate the expression patterns of proteins and growth factor signals in differentiated rabbit embryonic stem (ES) cells, ES cells with confluent stage grown of feeder layer and differentiated cells into embryoid bodies (EB) without feeder cell were applied to protein gel and Western blotting analysis. There were 66kDa and 28kDa specifically expressed in differentiated ES cell but not in undifferentiated ES cell while 25kDa protein band showed up in only undifferentiated ES cells. Also there were some difference of protein bands in several area of gel between differentiated and undifferentiated ES cells such as about 100 kDa, 50kDa and 27kDa areas, but there was no difference in band pattern of one-dimensional gel analysis between mouse ES cells and rabbit ES cells. IGF-I receptor and EGF receptor were expressed in differentiated cells and undifferentiated cells. And ICF-I and EGF were not expressed in both differentiated and undifferentiated cells. These results indicated that ES cells express their own proteins to inhibit differentiation while EB cells synthesize different proteins to differentiate, and 16F-I receptor and EGF receptor were expressed in both ES and EB cells probably for the different functions.
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KEYWORD
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Embryonic stem(ES) cell, Differentiation, Embryoid body (EB)
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